Somatic mutation analysis of p53 and ST7 tumor suppressor genes in gastric carcinoma by DHPLC

Table 2 p53 mutations in sporadic gastric carcinomas

Specimen No.	Pathology	Exon	Codon	Mutation	Effect	DHPLC (temp)	DHPLC gradient (B% in 4.5 min)
H3	Poorly diff.	6	188	CTG > CCG	Leu188Pro	63	60-66
54	Tubular	7	246	Del 24bpa	8 amino acid deletion	57,59,61	54-63
57	Tubular	6	215	AGT > GGT	Ser215Gly	62	57-66
64	Tubular	5	167	Ins 3bpa	Gln insertion	60	57-66
77	Tubular	7	235	Del 1bp(C)a	Framshift (246stop)	61	53-62
79	Papillary	8	301	CCA > CTA	Pro301Leu	60	54-63
86	Poorly diff.	5	161	GCC > GGC	Ala161Gly	61	53-62
133	Papillary	5	135	TGC > TGG	Cys135Trp	62	57-66
36		Intron 7		C > T, T > G		57	54-63

^aMutation that hasn’t been reported previously.