Expression of TNF-related apoptosis-inducing Ligand receptors and antitumor tumor effects of TNF-related apoptosis-inducing Ligand in human hepatocellular carcinoma

Figure 4 Transfection of pIRES-EGFP-TRAIL in vitro and in vivo. A: Transfected efficiency observed with fluorescence microscope (in vitro); B: The expression of TRAIL gene in nude mice tumor was all relatively strong (in vivo). (1), the expression of TRAIL gene in tissue near the tumor was weak (2) and that in normal liver tissue was negative after transfection of TRAIL gene (3); C: TRAIL protein production was determined by Western blot analysis. (a) Nude mice HCC tissue, (b) Adjacent tissue and (c) Normal tissue.