Deletion of Helicobacter pylori vacuolating cytotoxin gene by introduction of directed mutagenesis

Figure 2 Nucleotide sequence of cysS gene and the downstream sequence amplified from the vacA^-Km^r mutant H pylori. The 1398 bp cysS ORF and the 795 bp km^r ORF are shown. Primers la1, la2, ra1, ra2, and rs for amplifying LA, RA, and ASm are indicated. -35 signal, -10 signal, and rbs of vacA gene serving km ^r gene in the mutant strain are also shown.