Lethiferous effects of a recombinant vector carrying thymidine kinase suicide gene on 2.2.15 cells via a self-modulating mechanism

Figure 2 A: RT-PCR products of HCV IRES element. M: marker (2 kb), Lanes 1-3: HCV IRES element (330 bp). B. Component segment analysis of pcDNA₃-SCITK by agarose gel electrophoresis. M: marker (2 kb), Lanes 1-2: SCI-TK fragment demonstrated by PCR with HBV-S gene sense primer and TK antisense primer (5’-ACTTCCGTGGCTTCTTGCTG-3’ (nt 150-170). Lane 3: SCI fragment verified by PCR with HBV-S gene sense primer and HCV IRES antisense primer. Lane 4: SI segment from the ligation of HBV-S and HCV-Core gene by SOEing PCR. Lane 5: RT-PCR product of HCV-Core gene. Lane 6: Partial antisense segment of HBV-S gene.