Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli

Figure 2 Further identification of the antigen from mycoplasma infection. (A) 1. Western blot analysis of the total protein from Hela cells with MAb PD4, which was treated with cultured MGC803 medium. (A) 2. Western blot analysis of the total pro-tein from untreated HeLa cells with MAb PD4. (B) Western blot analysis of the total proteins from MGC803 cells treated with BM cyclin antibiotics differently. Lane 1: untreated, lane 2: treated for two weeks, and lane 3: treated for three weeks. As indicated by the arrow, the band reacted with MAb PD4 disappeared gradually following the treatment.