Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli

Figure 1 Purification of the antigen by MAb PD4. (A) 1. SDS-PAGE and Coomassie blue staining of the membrane proteins extracted from MGC803 cells; 2. Western blot analysis of the membrane proteins extracted from MGC803 with MAb PD4, the arrow indicating the target protein of MAb PD4. (B) 1. SDS-PAGE analysis of immunoprecipited protein complex bound with normal mouse IgG. (B) 2. SDS-PAGE analysis of immunoprecipited protein complex bound with MAb PD4. (B) 3. Western blot analysis of immunoprecipited protein complex bound with MAb PD4, arrow indicating the target protein of MAb PD4.