Gene expression profiles at different stages of human esophageal squamous cell carcinoma

Figure 1 Expression patterns of genes in esophageal tissue of normal, basal cell hyperplasia, high-grade dysplasia, carcinoma in situ, early and advanced cancer. Total RNAs were isolated from these tissues, reverse-transcribed into ³²P-labeled cDNAs and hybridized to Atlas^TM Human Cancer cDNA expression arrays. A complete list of names and location of the arrayed gene can be found in the instruction manual and website from Clontech. Data was analyzed by AtlasImage^TM 1.01 software. A, B, C, D, E, F showed hybridization result of normal cells, basal cell hyperplasia, high-grade dysplasia, carcinoma in situ, early and advanced cancer; G, H, I, J, K showed the color charts indicating up-regulated genes with red, down-regulated genes with blue, and non-changed genes with green in the later 5 stages when compared to normal tissue; L, M, N, O, P showed the pathological image of normal, basal cell hyperplasia, high-grade dysplasia, carcinoma in situ, early and advanced cancer respectively.