p73β inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73α

Figure 4 Effects of p73α and p73β on the regulatory sequences of hepatitis B virus. HepG2 cells were transfected with expres-sion plasmids, pcDNA3(1), p53(2), p73α (3), and p73β (4). In each case, 1 μg of reporter plasmids, pENI/XpLuc, containing the enhancer I/X promoter (A) and pENII/CpLuc, containing enhancer II/core promoter (B), were individually cotransfected. 42 hours after transfection, the cells were harvested and luciferase activity was determined. The value obtained with reporters alone was arbitrarily set to 100%, and the other values were normal-ized accordingly. The column heights reflect the average of at least three independent experiments.