Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease

doi:10.3748/wjg.v8.i6.1088

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Dec 15, 2002 (publication date) through Feb 9, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Dec 15, 2002; 8(6): 1088-1093
Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1088

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Figure 5 The inhibitory effects of PMSF and EDTA on single-chain protease activity. M, low molecular weight markers; lane1, single-chain serine protease; lane 2, protein substrate NS5ab; lane 3, without PMSF; lane 4, with 5 mmol/L PMSF; lane 5, without EDTA; lane 6, with 5 mmol/L EDTA.

Citation: Du GX, Hou LH, Guan RB, Tong YG, Wang HT. Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease. World J Gastroenterol 2002; 8(6): 1088-1093
URL: https://www.wjgnet.com/1007-9327/full/v8/i6/1088.htm
DOI: https://dx.doi.org/10.3748/wjg.v8.i6.1088