Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease

doi:10.3748/wjg.v8.i6.1088

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Dec 15, 2002 (publication date) through Sep 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Dec 15, 2002; 8(6): 1088-1093
Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1088

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Figure 1 Schematic diagram of constructs expressing single-chain serine protease NS3N4A and substrate NS5ab. His6, the hexahistidine metal chelation tag; GSVVIVGRIILS, NS4A central peptide (a.a. 21-32) and GSGS, an amino acid linker connecting the NS4A peptide to the NS3 serine protease domain. Cleavage site, representing the cleavage site between NS5A and NS5B.

Citation: Du GX, Hou LH, Guan RB, Tong YG, Wang HT. Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease. World J Gastroenterol 2002; 8(6): 1088-1093
URL: https://www.wjgnet.com/1007-9327/full/v8/i6/1088.htm
DOI: https://dx.doi.org/10.3748/wjg.v8.i6.1088