Retrovirus-mediated gene transfer of human endostatin inhibits growth of human liver carcinoma cells SMMC7721 in nude mice

Figure 3 Inhibition of endothelial cell proliferation by condi-tioned medium from SMMC-endo cells. Conditioned medium from SMMC-endo (2), SMMC7721 (3) and SMMC-pLncx (4) were concentrated and applied to HUVEC cells grown in 40-well plate. Three days later, cell number, as measured by absorbance(OD), was then quantified by using a colorimetric MTT assay. Bars, SD. bP<0.01, compared with conditioned medium from control SMMC-pLncx.