Expression of exogenous rat collagenase in vitro and in a rat model of liver fibrosis

Figure 4 Gelatin zymography analysis for the activity of collagenase. The enhanced gelatin degradation activity was found in the culture supernatant of pcmv-RC-F transfected cells (lane 1-7 represent the zymograph of 1:1, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64 diluted solutions of the supernatant) when compared with pTarge^TM transfected cells (lane 10, 11 represent the zymograph of 1:2 and 1:1 diluted solutions of the surpernatant). No activity was found at about M_r 55000 in the culture medium (lane 8, 9).