Establishment of a transgenic cell line stably expressing human cytochrome P450 2C18 and identification of a CYP2C18 clone with exon 5 missing

Figure 4 Representative chromatogram of extracts. A Shim-pack CLC-ODS column (15 × 0.6 cm i.d.) was used. The mobile phase was constituted with 0.05% phosphoric acid (pH2.6), acetonitrile (6:4/V:V) with the flow rate of 1 mL. min^-1. Hydroxytolbutamide was monitored at 230 nm. A: hydroxytolbutamide; B: tolbutamide