Establishment of a transgenic cell line stably expressing human cytochrome P450 2C18 and identification of a CYP2C18 clone with exon 5 missing

Figure 3 Electrophoresis and sequencing identification of a CYP2C18 clone with exon 5 missing. A: Electrophoresis identification of a CYP2C18 clone with an exon 5 being skipped; Lane 1: Marker (λ/EcoR I and Hind III); 2: Recombinant of pGEM-CYP2C18 digested by Nhe I and Xho I (incompleted digestion); B: Partial sequencing of an CYP2C18 cDNA clone with an exon 5 being skipped. The upper sequence represent the sense strand and the underside sequence represent the anti-sense strand being sequenced.