Establishment of a transgenic cell line stably expressing human cytochrome P450 2C18 and identification of a CYP2C18 clone with exon 5 missing

Figure 2 Electrophoresis identification of recombinants of pGEM-CYP2C18 and pREP9-CYP2C18. Lane 1: Marker (λ/EcoR I and Hind III); 2: pGEM-T vector; 3: Recombinant of pGEM-CYP2C18 digested by Nhe I and Xho I (incompleted digestion); 4: PCR products of CYP2C18 (1.67 kb); 5: Recombinant of pREP9-CYP2C18 digested by Nhe I and Xho I; 6: pREP9 vector.