Preparation of monoclonal antibody against apoptosis-associated antigens of hepatoma cells by subtractive immunization

Table 1 The fusion rates of hybridoma cells and the producing rates of antibodies

Group	Fusion wells/Incubatedwells (Fusion rate)	I (I/Fusion wells)	II (II/Fusion wells)	III (III/Fusion wells)	(I+II+III) ((I+II +III)/Fusion wells)
Experiment	241/384 (62.8%)	46 (19.1%)a	5 (2.0%)	0 (0%)	51 (21.2%)a
Control	262/384 (68.2%)	101 (38.5%)	4 (1.5%)	1 (0.4%)	106 (40.5%)

^aP < 0.01 vs Control. I: The wells whose antibodies reacted strongly with both apoptotic and non-apoptotic cells; II: The wells whose antibodies reacted strongly with apoptotic cells, but weakly with non-apoptotic cells (Difference is > 8 times); III: The wells whose antibodies reacted weakly with apoptotic cells, but strongly with non-apoptotic cells (Difference is > 8 times).