The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells

Table 1 Nucleotide sequence and size of the expected PCR products for oligonucleotide primers used for RT-PCR

Genes	Sequence	Size (bp)
c-jun	Upstream: 5'-GGAAACGACCTTCTATGACGAGCCC-3'	315
	Downstream: 5'-GAACCCCTCCTGCTCATCTGTCAGG-3'
β-actin	Upstream: 5'-GTGGGCCGCTCTAGGCACCAA-3'	540
	Downstream: 5'-CTCTTTGATGTCACGCACGATTTC-3'

The amplified products were seperated in 20 g•L^-1 agorose gel stained with ethidium bromide. After electrophoresis, the gel was observed and photographed under ultraviolet reflector. The density and area of each band were analyzed using ChemiImager^TM 4000 Digital System (Alpha Innotech Corporation, USA).