Stimulation of p38 MAPK by hormal preconditioning with atrial natriuretic peptide

Figure 4 ANP and JNK activity. Isolated perfused livers were either left untreated or preconditioned with ANP (200 nM) for 20 min. After 24 h of ischemia and up to 120 min of reperfusion JNK activity was determined as described under "Materials and Methods" Determination of density light units was performed by phosphorimaging and values for ANP-treated organs were divided by mean values for control organs at the respective time points. Panel A: Bars show means ± SEM of five independent perfusion experiments with ^aP < 0.05: statistically different from controls at the respective time point. Panel B: Data show representative autoradiograms.