Stimulation of p38 MAPK by hormal preconditioning with atrial natriuretic peptide

Figure 3 ANP increases the activity of p38 MAPK in the course of ischemia and reperfusion. Livers perfused with KH-buffer in the presence or absence of ANP (200 nM) underwent 24 h of cold ischemic storage and were snap frozen at the respective time points. p38 MAPK activity was determined as described under "Materials and Methods" Density light units were determined by phosphorimaging and values for ANP-treated organs were divided by mean values for control organs at the respective time points. Panel A: Bars show means ± SEM of five independent perfusion experiments with ^aP < 0.05 and ^bP < 0.01: statistically different from controls at the respective time point. Panel B: Data show representative autoradiograms.