Stimulation of p38 MAPK by hormal preconditioning with atrial natriuretic peptide

Figure 1 p38 MAPK activities in the course of ischemia and reperfusion. Livers were perfused through the portal vein with KH-buffer and snap frozen at 30 min, after 24 h of ischemic storage in UW solution (4 °C), and after 45 and 120 min of reperfusion. Deep-frozen organs were lysed and assayed for p38 MAPK activity as described under "Materials and Methods" Determination of density light units was performed by phosphorimaging and values for the respective time points are expressed as ration vs values for pre-ischemic (30 min) organs. Bars show means ± SEM of three independent perfusion experiments. ^aP < 0.05 and ^bP < 0.01: statistically different from pre-ischemic 30 min controls.