Full-length core sequence dependent complex-type glycosylation of hepatitis C virus E2 glycoprotein

Figure 3 Deglycosylation analysis of the expressed HCV E2 proteins with PNGase F. Transient expression products were subjected to Western blot analysis with M_E2116 as the primary antibody. pTM1 transfected cells were used as control. The plasmids used for transfection are indicated at the top of the lanes. Samples incubated with deglycosylation buffer were run in parallel. +: samples digested with PNGase F, -: samples incubated with PNGase F reaction buffer.