Full-length core sequence dependent complex-type glycosylation of hepatitis C virus E2 glycoprotein

Figure 2 Detection of E2 glycoprotein species of different molecular masses. Transient expression products were analyzed by Western blot with antibodies of distinct origin: mouse polyclonal antibody M_E2116 (lane 1, 2, 3); HCV patient serum S94 (lane 4, 5, 6); HCV patient serum S268 (lane 7, 8); rabbit polyclonal antibody R_E1135-C (lane 9, 10, 11). Empty vector pTM1 was used as the negative control. HCV-specific protein bands are indicated by arrowheads. The plasmids used for transfection are indicated at the top of the lanes.