The promoting molecular mechanism of alpha-fetoprotein on the growth of human hepatoma Bel7402 cell line

Figure 8 The effects of AFP on the expression of N-ras mRNA in Bel 7402 cells. 1 × 10⁵ cells were respectively treated with AFP (20 mg·L^-1), anti-AFP (40 mg·L^-1), AFP (20 mg·L^-1) + anti-AFP (40 mg·L^-1) or HSA (20 mg·L^-1) for 12 hours and expression of N-ras mRNA was detected by Northern blot assay. Lane 1: control group; Lane 2: HSA treated group; Lane 3: AFP treated group; Lane 4: anti-AFP treated group; Lane 5: AFP plus anti-AFP treated group. The data was selected from 3 independent experiments. A: Auto-radiograph of Northern blot. B: Quantitated by densitometric scanning of N-ras mRNA expression blot in Bel7402 cells (relative IOD units). The columns represent the means of triplicate determinations ± s.