The promoting molecular mechanism of alpha-fetoprotein on the growth of human hepatoma Bel7402 cell line

Figure 7 The effects of AFP on the expression of p53 (A) and p21 (B) proteins in Bel 7402 cells. 1 × 10⁵ cells were respectively treated with AFP (20 mg·L^-1), anti-AFP (40 mg·L^-1), AFP (20 mg·L^-1) + anti-AFP (40 mg·L^-1) or HSA (20 mg·L^-1) for 24 hours and the expression of p53 and p21protein were detected by Western blot assay. Lane 1: control group; lane 2: HSA treated group; lane 3: AFP treated group; lane 4: anti-AFP treated group; lane 5: AFP plus anti-AFP treated group. The data was selected from 3 indepen-dent experiments. A:p53; B:p21. The columns represent the means of triplicate determinations ± s.