Induction of apoptosis by TPA and VP-16 is through translocation of TR3

Figure 2 Translocation of TR3 protein from nucleus to cytosol in MGC80-3 cells. The cells treated with TPA for indicated time. (A) Translocation of TR3 protein observed by laser-scanning confocal microscope. TR3 protein was immunostained with anti-TR3 antibody and corresponding FITC-conjugated secondary antibody. (B) Western blot showed the translocation of TR3 protein. Nuclear and cytosolic fractions were prepared as described in materials and methods. α-tubulin was shown to quantify the loading protein. T: total protein.