Rapid screening mitochondrial DNA mutation by using denaturing high-performance liquid chromatography

Figure 1 The minimal ratio of hetero-: homoduplex in mixture were identi-fied by DHPLC. The composition of mutant type in PCR product mixture are 0% (A, wild type), 1% (B), 5% (C), 10% (D), 20% (E), 30% (F), 40% (G) and 50% (H) respectively. Chromatogram B has no difference to chromatogram A. Heteroduplex peak start to change in sample containing 5% variant (C). The heteroduplex can be obviously discerned in sample with 10% variant (D). Chromatogram E to H is almost similar. It indicates that about 5% mutant composition in PCR product mixture can be detected by DHPLC.