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©The Author(s) 2002.
World J Gastroenterol. Apr 15, 2002; 8(2): 276-281
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.276
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.276
Figure 3 Expression and intracellular location of preS1(21-119 aa) in E.
coli. BL21(DE3)plysS cells. Protein samples were subjected to 15% SDS-PAGE and stained with Commassia brilliant blue (A), and the duplicate gel was electroblotted onto a nitrocellulose membrane for western analysis (B). Lane M: molecular mass standards. Lane 1-2 are culture lysates of uninduced BL21 cells (lane 1) and induced BL21 cells (lane 2). Lane 3, 4: Supernatant (soluble) and precipitate (insoluble) fractions, respectively; Lane5: purified fusion protein by Ni2+-IDA-sepharose 6B.
- Citation: Wei J, Wang YQ, Lu ZM, Li GD, Wang Y, Zhang ZC. Detection of anti-preS1 antibodies for recovery of hepatitis B patients by immunoassay. World J Gastroenterol 2002; 8(2): 276-281
- URL: https://www.wjgnet.com/1007-9327/full/v8/i2/276.htm
- DOI: https://dx.doi.org/10.3748/wjg.v8.i2.276