JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential

Figure 4 The role of procaspase 8 and procaspase 9 in JTE-522 induced apoptosis for indicated time. 4A: Effect of JTE-522 on caspase activities for indicated time. Data presented are the mean value of three separate experiments. 4B: Western blot shows the cleavage of procaspase 8 (Casp 8) and procaspase 9 (Casp 9). AGS cells were treated with JTE-522 for the indicated times. 4C: Effect of caspase inhibitor on JTE-522 induced DNA fragmentation. AGS cells were treated with: 1: control; 2: 1 mmol/L JTE-522; 3: 0.5 mmol/L JTE-522; 4: 50 μmol/L Z-VAD.fmk + 1 mmol/L JTE-522; 5: 50 μmol/L Ac-IETD-CHO + 1 mmol/L JTE-522; 6: 50 μmol/L Ac-LEHD-CHO + 1 mmol/L JTE-522 for 24 h.