Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells

Figure 4 Agarose gel electrophoresis of difference products obtained after the first, second and third round hybridizations. BamH I-digested library DNA extracted from Alli823 and BGC823 cDNA libraries respectively (Lanes 2, 3); Amplicons of cDNA population prepared from Allitridi-treated and parental groups (used as tester and driver respectively) (Lane 4, 5); First, second and third difference products respectively (Lanes 6, 7, 8); 1 kb size marker (Lane 1) and PUC18/Hae III DNA size marker (Lane 9).