p16 gene methylation in colorectal cancers associated with Duke′s staging

Figure 1 Electrophoresis of MSP products of p16 CpG region. 1. 100 bp marker, 2. W control (142 bp), 3. M control (145 bp), 4. U control (154 bp), 5, 6, 7. The same sample (from patient No.9) reactive with W, M, U primers respec tively, 6. 145 bp product, 7. 154 bp product, indicating p16 was methylated in this sample, 8, 9, 10. Same sample (from patient No.34) reactive with W, M, U primers respectively, 10. 154 bp product, indicating that p16 was unmethylated in this sample.