Prokaryotical expression of structural and non-structural proteins of hepatitis G virus

Table 1 Amino acid location of constructed expression vector in HGV CH strain, yields in E. coli and immunoreactivity in Western blotting

Clone	Vector	Target fragments	Amino acid location in ORF	Molecular mass of recombinant protein (ku)	Yield in E. coli	Immuno-reactivity
G1	pGEX	Core to E1	1-144	42	High	+
G2	pRSET	E1 to E2	101-284	23	No
G3	pGEX	E2	247-578	62	No
G31	pGEX	E2	491-578	36	High	+
G4	pRSET	E2 to NS2	542-876	40	No
G5	pRSET	NS2 to NS3	854-1078	28	No
G6	pGEX	NS3	1073-1345	56	High	+
G61	pRSET	NS3	1160-1345	24	High	+
G7	pGEX	NS3	1267-1427	44	High	+
G8	pRSET	NS5	2151-2524	45	No
G81	pRSET	NS5	2151-2412	32	No
G82	pGEX	NS5	2408-2524	40	High	-
G821	pRSET	NS5	2357-2524	23	High	+
G61-821	pRSET	NS3+NS5	1160-1345+2357-2524	44	High	+