Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice

Table 4 Reversal of SMMC-7721 human hepatoma cell growth inhibition of AFP antisense S-ODNs by hybridization competition in vitro

Antisense (μmol/L)	Sense (μmol/L)	Hepatoma cell growth (absorbance at 570 nm)	Suppression (%)
0	0	1.00 ± 0.08
0	10	0.95 ± 0.07	5
10	0	0.46 ± 0.02	54
10	10	0.51 ± 0.05	49
10	100	0.95 ± 0.05	5

SMMC-7721 cells were incubated with combined AFP sense and antisense S-ODNs for 48 h in vitro. The cell growth suppression induced by antisense S-ODNs was reversed by hybridization competition of sense S-ODNs. Each value represents mean ± SD of 3 separate experiments.