Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes

Table 2 Effect of aminoguanidine, L-NAME and L-NNA on intracellular cGMP accumulation induced by CM in vitro

Groups	n	c (cGMP) (pmol·L-1)	Inhibition rate (%)
Control	7	1.29 ± 0.6
Cytokines mixture (CM)	12	3.01 ± 1.5a
CM + Aminoguanidine (0.1 mmol·L-1)	7	1.48 ± 0.8c	50.9
CM + L-NNA (0.1 mmol·L-1)	7	1.33 ± 1.1c	55.8
CM + L-NAME (0.1 mmol·L-1)	7	1.36 ± 0.4c	54.8

CM (Cytokines mixture): IL-1β 10 KU·L^-1, TNF-α 500 KU·L^-1, and IFN-γ 100 KU·L^-1 plus LPS 10 mg·L^-1; Cultured hepatocytes were stimulated in vitro with CM in the absence or presence of inhibitors (aminoguanidine, L-NAME, or L-NNA, respectively) for 24 h; Levels of intracellular cGMP were assayed 24 h after start of stimulation in vitro (CM or CM were co-incubated with inhibitors, respectively); ^-x±s, n = 7 rat or 12 rat (4 replicates for each treatment in each experiment); ^aP < 0.05 compared with control, ^cP < 0.05 compared with CM.