Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes

Table 1 Inhibitory effect of aminoguanidine and L-NAME on CM stimulated in vitro nitrite production in primary cultured rat hepatocytes

Dose (μmol·L-1)	n	c (NO2-) (μmol·L-1)	Inhibition rate (%)	Dose (μmol·L-1)	n	c (NO2-) (μmol·L-1)	Inhibition rate (%)
Control	6	6.2 ± 1.0		Control	6	6.5 ± 1.3
CM	16	12.3 ± 4.1b		CM	8	12.6 ± 3.7b
CM + Aminoguanidine				CM + Aminoguanidine
0.1	16	9.6 ± 3.9	22.0	0.1	9	10.1 ± 1.9	19.8
1	16	8.4 ± 2.6a	31.7	1	9	9.4 ± 2.5	25.4
10	16	6.9 ± 2.8d	44.0	10	9	8.5 ± 3.0a	32.5
100	16	5.7 ± 1.9d	53.7	100	9	7.7 ± 2.4a	38.9
1000	16	5.5 ± 2.1d	55.3	1000	9	7.8 ± 2.8a	38.1

CM (Cytokines mixture): IL-1β 10 KU·L^-1, TNF-α 500 KU·L^-1, and IFN-γ 100 KU·L^-1 plus LPS 10 mg·L^-1; Cultured hepatocytes were stimulated in vitro with CM in the absence or presence of inhibitors (aminoguanidine or L-NAME) at various concentrations for 24 h. Amounts of nitrite in the supernatant were assayed 24 h after start of stimulation in vitro. ^-x±s, n = 6-16 rats (3 well for each treatment in each experiment); ^bP < 0.01, compared with control, ^aP < 0.05, ^dP < 0.01 compared with corresponding CM.