Stress kinase inhibition modulates acute experimental pancreatitis

Figure 2 SB203580 inhibits cerulein-induced p38 kinase activation in vivo. Animals were treated with the indicated amounts of SB203580, sc, 2 h prior to cerulein hyperstimulation. In vitro phosphorylation of HSP27 following immunoprecipitation of MAPKAPK2 was used as read-out of p38 activity. 30 mg SB203580 almost completely abolished cerulein-induced pancreatic p38 activation.