Stress kinase inhibition modulates acute experimental pancreatitis

Figure 1 Effects of CEP1347 and SB203580 on acinar stimulus secretion coupling and trypsin activation. Acutely isolated acini were incubated with the indicated amounts of cerulein for 30 min (●). Acini were also treated with 20 μM CEP1347 (▽), 50 μM SB203580 (▼) or both agents simultaneously (○). A: Amylase release into the supernatant was determined and expressed as % of total content. Stimulus secretion coupling was not significantly altered by stress kinase inhibitors. B: Active trypsin was measured in acinar homogenates and expressed as pg/mg protein. Although SB203580 treated acini had a tendency towards higher amounts of active trypsin, this effect was not statistically significant.