Copyright
©The Author(s) 2000.
World J Gastroenterol. Aug 15, 2000; 6(4): 546-552
Published online Aug 15, 2000. doi: 10.3748/wjg.v6.i4.546
Published online Aug 15, 2000. doi: 10.3748/wjg.v6.i4.546
Figure 1 Expression of CD45 and ANK61 antigen on hepatic NK cells (pit cells).
The cells were washed out of the liver by sinusoidal lavage. After Ficoll-Paque gradient centrifugation and nylon-wool adherence to remove erythrocytes, granul ocytes, monocytes and B lymphocytes, the cells were stained with anti-NKR-P1A mAb (3.2.3) (phycoerythrin) and anti-ANK61 antigen (ANK61), anti-CD45 mAb (ANK 74) (fluorescein) and analyzed by two-color flow cytometry. CD45 and ANK61 anti gen were expressed on the x-axis (fluorescein), NKR-P1A on the y-axis (phycoe rythrin).
- Citation: Luo DZ, Vermijlen D, Ahishali B, Triantis V, Vanderkerken K, Kuppen PJ, Wisse E. Participation of CD45, NKR-P1A and ANK61 antigen in rat hepatic NK cell (pit cell)-mediated target cell cytotoxicity. World J Gastroenterol 2000; 6(4): 546-552
- URL: https://www.wjgnet.com/1007-9327/full/v6/i4/546.htm
- DOI: https://dx.doi.org/10.3748/wjg.v6.i4.546