Construction of HBV-specific ribozyme and its recombinant with HDV and their cleavage activity in vitro

Figure 3 Cleavage of target RNA (rBVCF) catalyzed by rRZ and rDVRZ. a: Cleavage of target RNA (rBVCF) by rRZ under 37 °C, 42 °C and 55 °C with 20 mmol/L Mg²⁺. b: Cleavage of target RNA (rBVCF) by rDVRZ under 37 °C, 42 °C and 55 °C with 20 mmol/L Mg²⁺. c: Cleavage of target RNA (rBVCF) separately by rRZ and rDVRZ at 37 °C under different Mg²⁺ concentrations (10 mmol/L, 15 mmol/L) (Lane 1, 2: cleavage by rRZ separately under 10 and 15 mmol/L Mg²⁺; Lane 3, 4: cleavage by rDVRZ separately under 10 and 15 mmol/L Mg²⁺)