Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes

Table 1 Details of antisera and main reagents used

Antisera against	Working dilution	Code	Specificity	Source
Synthetic human gastrin	1:5000	GP-1304	No cross reaction with cholecystokinin-8	Dr. N Yanaihara,Shizuoka
Leucine-enkephalin	1:80000	1671	—	UCB-Bioproducts Bruxelles
Bovine pancreatic polypeptide	1:12000	615-R-110	Cross-react s with human pancreatic polypeptide	Dr. RE Chance, Indianapolis
Substance P	1:2000	MAS 035B	—	Sera-Lab., Sussex
Gastric inhibitory polypeptide	1:10000	G/R/34-IIID	No cros s reaction with glucagon	Dr. D Grube, Hannove
Porcine glucagon	1:1000	RPN1602	Wholly cross react with pancreatic	Amersham International pl.,
			and intestinal glucagon	Amersham
Synthetic human cyclic
somatostatin	1:3000	—	—	Dr. S Ito Niigata
Glucagon-like immunoreactants	1:1000	RPN1604	Wholly cross react with pancreatic	Amersham International pl.,
			and intestinal glucagon	Amersham
Insulin	1:1000	47291	—	—
Avian pancreatic polypeptide	1:10000	Iance-10/5/81	No cross reaction with glucagon	Dr. JR Kimmel, Kansas City
5-Hydroxytriptamine	1:10000	Lot.16302	—	Immunonuclear Corp.Stillwater
Synthetic porcine motilin	1:1000	R-1104	Reacts against entire molecules	Dr. N Yanaihara, Shizuoka
Natural porcine
cholecystokinin-33	1:3000	—	Reacts with cholecystokinin 11-20,	Dr. J Yamada, Shizuoka
			no cross reaction with gastrin
Synthetic porcine secretin	1:1000	R-801	Reacts with the C- and N-terminals	Dr. N Yanaihara, Shizuoka
Synthetic bovine neurotensin	1:1000	R-3501		Dr. N Yanaigar a, Shizuoka
Synthetic porcine vasoac tive
intestinal polypeptide	1:2000	R-502	Reacts against entire molecules	Dr. N Yanaihara, Shizuoka
Bombesin	1:3000	27070	Cross reaction with GRPImmunonrclear	Corp. Stillwater
Neuron specific enolase	1:1000	—	—	Dako, Copenharge
Prochymosin	1:2000	—	—	A. Andren, Uppsala
Pepsinogen	1:2000	—	—	A. Andren, Uppsala
Rabbit PAP	1:100	Z - 113	—	Dako, Copenharge
Guineapig PAP	1:50	24699	—	Dako, Copenharge
PAP Kit	1:100	61- 2003	—	Zymed Lab. Inc., SouthSan
				Francisco, USA
ABC Kit	1:50	PK-4001	—	Vector Lab. Burlingame USA