A DNA delivery system containing listeriolysin O results in enhanced hepatocyte-directed gene expression

Figure 3 Targeted β-galactosidase gene expression. Conjugates containing 1μg of β-gal DNA were added to Huh7 (ASG receptor positive) or SK Hepl cells (ASG receptor negative) and incubated for 48 h. Cells incubated with â-gal were washed with PBS pH 74, fixed with 4% paraf ormaldehyde and stained with X-gal as described in Materials and Methods. Cells were observed with light microscopy and positive (blue stained) cells were coun ted. ASOR-PL-DNA, lane 1; ASOR-PL-LLO-DNA, lane 2; ASOR-PL-LLO-DNA+ASOR, lane 3; ASOR-PL-DNA+LLO, lane 4; DNA alone, lane 5.