HBeAg gene expression with baculovirus vector in silk worm cells

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Apr 15, 1999 (publication date) through Dec 1, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 15, 1999; 5(2): 167-171
Published online Apr 15, 1999. doi: 10.3748/wjg.v5.i2.167

Table 3 Purification of HBeAg Produced by Bm N cells

Method	Total activity (by ELISA)	Specific activity (per mg protein)	Purification factor		Total recovery (%)
Method	Total activity (by ELISA)	Specific activity (per mg protein)	A	B	Total recovery (%)
Cell culture medium	1:1.60 × 10⁶	1:2.9 × 10⁵	1.0	/	/
(NH4)₂SO₄	1:1.32 × 10⁶	1:8.5 × 10⁵	2.9	2.9	80
Sephacryl S-200	1:1.12 × 10⁶	1:5.3 × 10⁴	6.3	18.3	70
DEAE Sepharose FF	1:8.40 × 10⁵	1:5.0 × 10⁵	9.4	172.0	52

A: Individual steps; B: Accumulated results.

Citation: Deng XZ, Diao ZY, He L, Qiao RL, Zhang LY. HBeAg gene expression with baculovirus vector in silk worm cells. World J Gastroenterol 1999; 5(2): 167-171
URL: https://www.wjgnet.com/1007-9327/full/v5/i2/167.htm
DOI: https://dx.doi.org/10.3748/wjg.v5.i2.167