Detection of bacterial DNA from cholesterol gallstones by nested primers polymerase chain reaction

Figure 1 Agarose gel electrophoresis comparative analysis of NP-PCR products of bacterial DNA fragments from gallstones. M: Molecular standard, Lambda DNA/EcoR I + Hind III. Lanes 1, 2, 3, 6, 8 and 10: NP-PCR positive products similar to the amplified products of 11 P.acnes 16S rRNA gene; 11: amplified product of P.acnes 16S rRNA gene; 7, 9: NP-PCR positive products, molecular weight less than 300 bp; 4: negative control; 5: NP-PCR negative product.