Cloning of 3H11 mAb variable region gene and expression of 3H11 human-mouse chimeric light chain

doi:10.3748/wjg.v4.i1.41

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Feb 15, 1998 (publication date) through Jul 31, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 15, 1998; 4(1): 41-44
Published online Feb 15, 1998. doi: 10.3748/wjg.v4.i1.41

Table 2 Expression of chimeric light chain in transfectants determined by ELISA

ELISAsystem	Supernatant			Human Ig	Mouse Ig
ELISAsystem	3H11	Sp2/0	Transfectant	Human Ig	Mouse Ig
Anti-human	0.169	0.112	1.214	1.660	0.122
Anti-mouse	1.564	0.147	0.128	0.102	1.538

a. Anti-human Fab and HRP-anti-human Fab Ab were used as catcher and second Ab. b. Anti-mouse Fab and HRP-anti-mouse Fab Ab were used as catcher and second Ab. c. ABS492.

Citation: Li J, Wang Y, Li QX, Wang YM, Xu JJ, Dong ZW. Cloning of 3H11 mAb variable region gene and expression of 3H11 human-mouse chimeric light chain. World J Gastroenterol 1998; 4(1): 41-44
URL: https://www.wjgnet.com/1007-9327/full/v4/i1/41.htm
DOI: https://dx.doi.org/10.3748/wjg.v4.i1.41