Cloning of 3H11 mAb variable region gene and expression of 3H11 human-mouse chimeric light chain

doi:10.3748/wjg.v4.i1.41

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Feb 15, 1998 (publication date) through Dec 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 15, 1998; 4(1): 41-44
Published online Feb 15, 1998. doi: 10.3748/wjg.v4.i1.41

Table 1 PCR primers for cloning variable genes of rearranged immunoglobulin light and heavy chains

5’Primers for VL Signal peptide

LL1: GGGGATATCCACCATGGAGACAGACACACTCCTGCTAT

LL2: GGGGATATCCACCATGGATTTTCAAGTGCAGATTTTCAG

LL3: GGGGATATCCACCATGGAGWCACAKWCTCAGGTCTTTRTA

LL4: GGGGATATCCACCATGKCCCCWRCTCAGYTYCTKGT

LL5: GGGGATATCCACCATGAAGTTGCCTGTTAGGCTGTTG

3’Primer for VK spanning CK and JK1,2&4

MVK: GGATACAGTTGGTGCAGTCGACTTACGTTTKATTTCCARCTT

5’Primers for VH signal peptide

VHL1: GGGGATATCCACCATGGRATGSAGCTGKGTMATSCTCTT

VHL2: GGGGATATCCACCATGRACTTCGGGYTGAGCTKGGTTTT

VHL3: GGGGATATCCACCATGGCTGTCTTGGGGCTGCTCTTCT

VHL4: GGGGATATCCACCATGATRGTGTTRAGTCTTYTGTRCCTG

3’Primer for VH spanning CH1 and JH

MVH: GACHGATGGGGSTGTYGTGCTAGCTGNRGAGACDGTGA

Citation: Li J, Wang Y, Li QX, Wang YM, Xu JJ, Dong ZW. Cloning of 3H11 mAb variable region gene and expression of 3H11 human-mouse chimeric light chain. World J Gastroenterol 1998; 4(1): 41-44
URL: https://www.wjgnet.com/1007-9327/full/v4/i1/41.htm
DOI: https://dx.doi.org/10.3748/wjg.v4.i1.41