Oxidative stress-induced circSOD2 inhibits osteogenesis through sponging miR-29b in metabolic-associated fatty liver disease

Figure 5 circSOD2 sponges miR-29b. A: Reverse transcription-quantitative polymerase chain reaction (qRT-PCR) analysis of argonaute-2-bound circSOD2 using RNA immunoprecipitation; B: Venn diagram illustrating 12 potential target miRNAs of circSOD2 through cross-referencing TargetScan, RNAbybrid, and miRanda databases; C: Quantification of the efficiency of the circSOD2 probe for RNA antisense purification analysis using qRT-PCR; D: Quantification of the circSOD2-bound miRNAs using qRT-PCR; E: Relative luciferase activity of circSOD2 Luciferase reporter plasmids co-transfected with candidate miRNA mimics in HEK-293T cells; F: Quantification of relative luciferase activity using dual luciferase reporter assay after co-transfection of Luc-circSOD2 WT or Mut with miR-29b mimic or negative control in HEK-293T cells; G: Fluorescence in situ hybridization assay images demonstrating co-localization of circSOD2 and miR-29b in the cytoplasm; H: Decreased expression of miR-29b in bone marrow mesenchymal stem cells from high-fat diet mice (n = 5). ^aP < 0.05; ^bP < 0.01; NS: Not significant; NC: Negative control; AGO2: Analysis of argonaute-2; RIP: RNA immunoprecipitation; WT: Wildtype; Mut: Mutation; Con: Control.