Oxidative stress-induced circSOD2 inhibits osteogenesis through sponging miR-29b in metabolic-associated fatty liver disease

Figure 1 circSOD2 is upregulated in metabolic-associated fatty liver disease. A: Heat map depicting differentially expressed circRNAs in the liver from three clinical subjects with metabolic-associated fatty liver disease (MAFLD) and three control subjects after RNA sequencing; B: Validation of the top 10 upregulated circRNAs in MAFLD livers, including circSOD2 (hsa_circ_0004662), confirmed using quantitative real-time reverse transcription polymerase chain reaction (n = 5); C and D: CircSOD2 was highly expressed in primary hepatocytes and serum subjects with MAFLD (n = 5); E: RNase R digestion assay showing that circSOD2 was more resistant to degradation by RNase R; F: Fluorescence in situ hybridization assay showing that circSOD2 was predominantly located in the cytoplasm. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; NS: Not significant; Con: Control; NAFLD: Nonalcoholic fatty liver disease.