Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation

Figure 9 Runchangningshen paste changed the mucosal microbiota function to promote mucin-2 secretion in functional constipation rats. A: Heatmap of Kyoto Encyclopedia of Genes and Genomes database (KEGG) functional enrichment of differential microbiota between normal control group (NC), functional constipation model group (FC) and high dose of runchangningshen paste (HRCNSP) groups at the pathway level 1 (n = 6); B: Heatmap of KEGG functional enrichment of differential microbiota between NC, FC and HRCNSP groups at the pathway level 2 (n = 6); C: Heatmap of KEGG functional enrichment of differential microbiota between NC, FC and HRCNSP groups at the pathway level 3 (n = 6); D: Orthologous groups of proteins database functional classification diagram of differential microflora among NC, FC, and HRCNSP groups (n = 6). In Figures A-C, the x-axis represents the group names, while the y-axis indicates the functional names at pathway levels 1/2/3. The color gradient of the blocks illustrates the variations in functional abundance across the groups, with the legend indicating the values represented by the color gradient. In Figure D, different colors represent different functional classifications, the x-axis represents the relative abundance of functional classifications, and the y-axis represents groups. COG: Cluster of Orthologous Groups.