Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation

Figure 6 Runchangningshen paste changed the gut mucosal microbiota diversity in functional constipation rats. A: Pan curve; B: Core curve; C: Rank-Abundance curves; D-G: Alpha diversity analysis. The y-axis represents the operational taxonomic unit (OTU) level corresponding to each group based on the different indexes (n = 6) (D: Simpson index analysis; E: Shannon index analysis; F: Chao index analysis; G: Ace index analysis); H-M: Beta diversity analysis. The x-axis and y-axis represent two selected principal component (PC1 and PC2) axes, while the percentages indicate the explanatory values of the principal components regarding the differences in sample composition. Stress is used to assess the quality of the Non-metric multidimensional scaling analysis (NMDS) analysis results. Different colors or shapes represent different samples or groups. The closer the sample points or shapes are, the more similar the species composition of the two or two groups of samples becomes (n = 6) [H: Principal coordinates analysis (PCoA); I: Principal component analysis (PCA); J: NMDS analysis; K: Partial least squares discriminant analysis (PLS-DA); L: ANOSIM analysis; M: Kruskal-Wallis; H test analysis). All data were shown as the mean ± SD. P values were calculated using the ANOSIM test. ANOVA: Analysis of variance; FC: Functional constipation model group; HRCNSP: High dose of runchangningshen paste group; LAC: Lactulose group; LRCNSP: Low dose of runchangningshen paste group; MRCNSP: Medium dose of runchangningshen paste group; NC: Normal control group.