Modified Pulsatilla decoction alleviates 5-fluorouracil-induced intestinal mucositis by modulating the TLR4/MyD88/NF-κB pathway and gut microbiota

Figure 9 Modified Pulsatilla decoction ameliorates gut microbiota dysbiosis. A and B: Amplicon sequence variant (ASV) Venn diagram (A) and counts (B); C and D: Stacked bar charts of relative species abundance at genus (C), and species (D) levels; E-J: Alpha diversity indices including observe ASV (E), ACE (F), Chao1 (G), Shannon (H), Simpson (1-D; I), and Pulsatilla decoction whole tree (J); K-P: Beta diversity analysis of Unweighted PCoA (K), Unweighted PCoA with UPGMA (L), Weighted PCoA (M), Weighted PCoA with UPGMA (N), Bray-Curtis PCoA (O) and NMDS (P). ^aP < 0.01 vs control group; ^bP < 0.05, ^cP < 0.01 vs 5-fluorouracil group. ASV: Amplicon sequence variant; LO: Loperamide; PD: Pulsatilla decoction.