Modified Pulsatilla decoction alleviates 5-fluorouracil-induced intestinal mucositis by modulating the TLR4/MyD88/NF-κB pathway and gut microbiota

Figure 6 Modified Pulsatilla decoction exerts inhibitory effect on TLR4/MyD88/NF-κB pathway. A and B: Western blotting images of TLR4, MyD88, TRAF6, β-actin (A), and NF-κB P65, Histone3 (B); C-F: Ratios of TLR4/β-actin (C), MyD88/β-actin (D), TRAF6/β-actin (E), and P65/Histone3 (F); G-N: Immunofluorescent images for TLR4 (G), MyD88 (I), TRAF6 (K), and NF-κB (M) with corresponding fluorescence intensities (H, J, L and N), normalized to the control group (set as 1). Data are shown as mean ± SD (n = 3). ^aP < 0.01 vs control group; ^bP < 0.01 vs 5-fluorouracil group. 5-FU: 5-fluorouracil; LO: Loperamide; PD: Pulsatilla decoction.